en Nursing Orginal research <strong><em>Background:</em></strong> Peer education is one of the active teaching methods and is rarely used in clinical settings, especially in nursing.<br> <strong><em>Objectives: </em></strong>This study aimed to investigate the effects of Peer Assisted Learning (PAL) on learning the principles of vaccination in nursing students.<br> <strong><em>Methods:</em></strong> This quasi-experimental study was performed on 79 (intervention group n=43, control group n=36) nursing students. In the intervention group, seven peer instructors taught the principles of vaccination in two one-hour sessions to other students. The control group received routine education. Data were collected using a questionnaire<span dir="RTL">.</span><br> <strong><em>Results:</em></strong> The findings showed that among the students, 43% (n=34) were male and 57% (n=45) were female participants. The difference between the intervention and control groups was significant after the intervention (P<0.001). Besides, the comparison of the average score of the checklist between the two groups showed significant differences (P<0.001).<br> <strong><em>Conclusion:</em></strong> The results of this study showed the positive impact of PAL on clinical education as well as its reliability and benefits for nursing students<span dir="RTL">.</span> learning, nursing, peer group, students, vaccination 21 26 http://nmcjournal.zums.ac.ir/browse.php?a_code=A-10-724-1&slc_lang=en&sid=1 Malihe Abootalebi maliheabootalebi@gmail.com 10031947532846007407 10031947532846007407 No University of Social Welfare and Rehabilitation Sciences, Tehran, Iran Naval Heydari navalheydari@gmail.com 10031947532846007408 10031947532846007408 No Student Research Committee, Department of Nursing, School of Nursing and Midwifery, Shiraz University of Medical Sciences, Shiraz, Iran Fatemeh vizeshfar vizeshfarf@sums.ac.ir 10031947532846007409 10031947532846007409 Yes Department of Nursing, School of Nursing and Midwifery, Community Based Psychiatric Care Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Maryam Paran mparan90@gmail.com 10031947532846007410 10031947532846007410 No Department of Nursing, School of Nursing and Midwifery, Shiraz University of Medical Sciences, Shiraz, Iran